2021-7-9 · ClonExpress II One Step Cloning Kit 25 rxn. Home » Shop » ClonExpress II One Step Cloning Kit 25 rxn. ClonExpress® technology is a simple fast and efficient DNA seamless cloning technology which can clone the insert into any position of any vector. Linearize the vector and place the insert 5 in the forward/reverse PCR primer Introduce
ClonExpress II One Step Cloning Kit C112-02 -Equl_AdvancedBioMatrix_DivBio_Drummond_Genie_Glascol_Megazyme_Phadebas_Worthington Megazyme AUVON ElisaSystems Phadebas Phadebas EBPI TDzyme
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector self-ligation and the positive rate can reach more than 95 . The highly optimized reaction buffer and the enhanced recombinase Exnase II significantly improve the recombination efficiency of
2020-2-3 · PCR products were inserted into lined pART27 GFP using ClonExpress II One Step Cloning Kit (Vazyme China). These positive clones were transformed into Agrobacterium tumefaciens strain GV3101. The pART27 GFP transformed Agrobacterium
2021-3-15 · C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
ClonExpress II One Step Cloning Kit. 73.00 €122.00 €. excl. VAT plus shipping. Size. Choose an option 25 RXNS 50 RXNS. Clear. ClonExpress II One Step Cloning Kit quantity. Add to cart. Categories Cloning Mutagenesis Fast Cloning Molecular Biology SKU C112.
ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
The purified PCR fragments were ligated with the linearized plasmid pMA5-sat via Gibson assembly using the ClonExpress II One-Step Cloning Kit and the resultant plasmid was used to transform competent E. coli JM109 cells. The recombinant plasmids
2014-4-27 · ClonExpress TM II 500 bp control insert (25 ng/μl) pUC19 control vector linearized (50 ng/μl) μl 1 (
2021-7-20 · ClonExpress ® DNA ClonExpress ® II Exnase ® II
As a new generation recombinant cloning kit ClonExpress® II has a unique non-ligase dependent system which greatly reduces the background of vector
ClonExpress II One Step Cloning Kit Vazyme 25 rxn/50 rxn C112-01/02 600/1000
Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One Stepo Qrt Pcr Igg Igm Oneblood Antibodies Test Anti Bodies Buy Antibodies Virus Antibodies Only 23 Months Anti Human Only Antibodies Raised In Goats
2021-6-16 · ClonExpress®II Exnase®II / PCR 5 PCR 5 3 (15 bp-20 bp)
2017-5-12 · The assembled fragment was inserted to the linearized pRGEB32 using ClonExpress® II One Step Cloning Kit (Vazyme Nanjing China) thus generating pRGEB32-GhU6.9 vector. It has a hpt selection marker and was used to target a reporter gene DsRed2 which was firstly transformed into cotton with a vector carrying NPT II (Neomycin
2021-7-22 · ClonExpress II One Step Cloning Kit C112 Vazyme 6001000 ClonExpress MultiS One Step Cloning Kit
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2016-3-2 · ClonExpress® II 0.03 pmol 1 2 0.06 pmol DNA = 0.02 ng (0.03 pmol)
2021-3-15 · C112 ClonExpress II One Step Cloning kit.pdf Created Date 6/5/2019 1 12 08 PM
ClonExpress-II One Step Cloning Kit (Vazyme Nanjing China) respectively. For fatty acids analysis in yeast HMS1 and HMS1I were cloned into pYES2 and pRS416 vectors respec-tively. All primers are shown in Supporting Information Table S1. Expression analyses Total RNA was isolated from rice anthers or spikelets using Tri-zol reagent (Invitrogen).
2021-6-18 · ClonExpress ® II Exnase ® II PCR5 - PCR5 3 15bp 20bp
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2014-4-27 · ClonExpress TM II 500 bp control insert (25 ng/μl) pUC19 control vector linearized (50 ng/μl) μl 1 (
ClonExpress® II One Step Cloning Kit. . 95 . 50 bp-10 kbp DNA. PCR. .
ClonExpress II One Step Cloning Kit and Phanta Max (p515) DNA polymerases were purchased from Vazyme Biotech Co. Ltd. (Nanjing China). Oligonucleotides were synthesized by GENEWIZ Bio Inc. (Suzhou China). Strains plasmids and media. The strains and plasmids involved in this study are listed in table S3. The MP6 and CRISPR systems for gene
Clonexpress Ii One Step Cloning Ki Clonexpress Ii One Step Cloning Kit Clonexpress Ii One Step Cloning Kit Cells Direct One Step Qrt Pcr Kit Accurate Chemicals One Step Cell Direct One Stepo Qrt Pcr Igg Igm Oneblood Antibodies Test Anti Bodies Buy Antibodies Virus Antibodies Only 23 Months Anti Human Only Antibodies Raised In Goats
2016-3-2 · ClonExpress® II 0.03 pmol 1 2 0.06 pmol DNA = 0.02 ng (0.03 pmol)
2016-8-5 · ClonExpress II One Step Cloning Kit (Vazyme Biotech Co. Ltd). Typically 20 μL of a mixture containing 4 μL of 5 CE II Buffer 2 μL of Exnase II 50 ng linear plasmid 100 ng PCR fragment and ddH2O was incubated at 37oC for 30 min and the resulting solution was used to transform chemically competent E. coli DH5α cells. Colony PCR and
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